I'm trying to do a high-throughput screening approach using yeast grown in 384-well plates. As the yeast grow, they tend to fall to the bottom of the plate, leaving a pellet with a clear supernatant on top. I'd like to resuspend them so I can replicate the plates with our replicating robot (Genetix qPix2).
The pins on the replicating robot do not reach the bottom of the 384 well plate. I'm looking for a simple way to re-suspend these cultures. I have a plate-shaker (Barnsted titer plate shaker) that doesn't seem to do the trick. I have ~ 100 plates so manual pipetting is not an option.
I have access to a BioMek across campus as a last resort, but I think there should be some way to resuspend them otherwise.
Let me know if you have any thoughts!