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Resuspending cells in 384-well plate

high throughput 384 well plate

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#1 kmewis

kmewis

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Posted 24 September 2013 - 03:57 PM

Hey all,

 

I'm trying to do a high-throughput screening approach using yeast grown in 384-well plates. As the yeast grow, they tend to fall to the bottom of the plate, leaving a pellet with a clear supernatant on top. I'd like to resuspend them so I can replicate the plates with our replicating robot (Genetix qPix2). 

 

The pins on the replicating robot do not reach the bottom of the 384 well plate. I'm looking for a simple way to re-suspend these cultures. I have a plate-shaker (Barnsted titer plate shaker) that doesn't seem to do the trick. I have ~ 100 plates so manual pipetting is not an option.

 

I have access to a BioMek across campus as a last resort, but I think there should be some way to resuspend them otherwise.

 

Let me know if you have any thoughts!






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