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T cell stimulation with peptides - how does it work?

immunology elisa peptide presentation elispot

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#1 hasebe

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Posted 24 September 2013 - 03:34 PM

Hi,

 

I searched the forums and basically I have the same question someone else had in this thread: http://www.protocol-...s-in-ics-assays . So maybe somebody can help?

 

I was wondering why in some assays, T cells and their TCRs do not require MHC-bound antigen and are stimulated only by 'free' peptides. In my books, both CD4+ and CD8+ T cells require peptide/MHC complexes for epitope recognition, so in theory this shouldn't work without APCs or at least multimers.

 

For example, in Elispot assays peptide stimulation can be used as a positive control, like shown on this page: http://www.mabtech.c...001004&MenuId=7 . At first I thought that APCs might take up the peptide and present it to the T cells. But then I discovered that T cells are stimulated even when there are no APCs around (enriched CD8+ cells or T cell clone), as is the case in the above link.

 

 

Can someone explain? Thanks for your help!







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