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Weighing harvested cells


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#1 robradford

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Posted 24 September 2013 - 07:06 AM

Hi Folks,

 

I've a bit of an odd question. A collaborator of mine needs 100mg of cells for an experiment he is about to run, so I'm providing the cells.

 

I just wanted to ask if anyone has had any experience with how many T-175 flasks would be necessary for this (I'm using IMR90 human fibroblasts).

 

The last time I split my cells I found I got nearly 50 mg from one almost confluent T-175, but I'm more than a bit skeptical of this number, so any input would be greatly appreciated! 

 

Thanks,

 

Rob



#2 jerryshelly1

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Posted 24 September 2013 - 01:26 PM

Figure out your OD. You could easily calculate "mg" of cells from this number.






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