I have a question about the effect of using FBS during kinetic of inhibition using inhibitors of signalling pathways. For some of the inhibitors I use, I have a little effect on the protein rates I study and some collegues of mine said that FBS in the culture medium could "hide" part of the effect... Did you hear or face such a problem? Is that a matter of cytokines and growth factors that could activate the pathway that is targeted?
Another question would be : what other conditions can modify the effect of such inhibitions?
Than you in advance for your help.