I am very new to real time PCR. I need to quantify some citokynes by real time PCR with sybr green for mouse cytokines. (the genes are IL-1b, IL-6, IL-8, TNF alpa and COX-2.
Since it's plenty of primers out there, the choice is not easy. Even I found some publications regarding that I couldn't find the relevant bp values.( For real time PCR ,I heard the bp should be low and the maximum is below 400bp)
Someone has any experience to tell me with any published primer for these cytokines.
Thanks for any suggestion.