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Gene sequence for Real time PCR

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#1 sadaru

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Posted 23 September 2013 - 09:09 AM

I am searching gene sequence for real time PCR. I couldn't get the data for it. I need to get gene sequences for mouse TNF-alpha, IL-1beta........ for real time PCR. I searched many SCI journals and I found many are using qPCR or rats' gene sequences. I need to get  200-400bp sequences and i couldn't find it. Please some one help me to show the way.

 

Thank you in advance. 



#2 Epigeneticist

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Posted 07 October 2013 - 05:05 PM

Why don't you go to NCBI GenBank to retrieve the ref gene sequence or the cDNA? If not, could you please explain your question differently?



#3 Tabaluga

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Posted 13 October 2013 - 08:15 AM

Do you mean primers by chance and not sequences of the genes themselves ?


Il dort. Quoique le sort fût pour lui bien étrange,
Il vivait. Il mourut quand il n'eut plus son ange;
La chose simplement d'elle-même arriva,
Comme la nuit se fait lorsque le jour s'en va.

 






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