I have a bunch of electron microscopy pictures taken at the same magnification and I need to calculate the area of some organelles. I know how to use ImageJ for this purpose. However, I'm having the following problem.
When I try to measure the area objects of the same size in different pictures I get completely disparate results. I assume this has to do with the resolution of the picture and some sort of conversion that ImageJ does, because since the pictures were taken with the same magnification, the area must be identical. I tried to use the "Analyze>Set Scale" option, but unsuccessfully. I don't have a scale bar in my pictures.
Can someone help me??
Thank you in advance.
Edited by cardosopedro, 23 September 2013 - 05:34 AM.