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Labeling DNA with Biotin

Bio 11-dUTP or Bio 16-dU

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#1 theo22

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Posted 23 September 2013 - 01:20 AM

Hello,

 

I would like to label some DNA wth Biotin by PCR. I can buy two kind of Biotin labeled dUTP nucleotides: Bio-11-dUTP and Bio-16-dUTP. Which one do I need?

 

Thanks,

 

Theo



#2 phage434

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Posted 23 September 2013 - 06:31 AM

Either is fine. The -16 has a slightly longer linker, which may make it a bit easier to bind in some downstream applications. It probably does not matter.

Don't forget you need to use Taq or a Taq + Pfu like mixture for PCR to incorporate the dUTP.



#3 theo22

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Posted 23 September 2013 - 07:01 AM

Thanks!






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