I have to clone a gene which shows "....mRNA, complete cds" on PUBMED.
Now I need to find out the target sequence and design a primer for cloning this gene.
I know I would have to extract the RNA and convert it to cDNA during isolation.
However, can I directly use this "....mRNA, complete cds" to pick primers in blast? Or do I need to pick primers in the mRNA sequence?
Thank you very much for your help.
Edited by helpwithdna, 22 September 2013 - 11:14 PM.