Does anyone know what medium should be used to set up GH teratocarcinoma cell line? I tried to look this information up in several publications which forwarded me to other resources but the search proved unsuccessful each time. I also contacted several companies that sell different cell lines and they could not help me either.
I tried growing them in RPMI 1640 with FCS and Pen/Strep last week but they died on day 3.
Any advice would be highly appreciated.