Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Growing GH teratocarcinoma cell line- help needed


  • Please log in to reply
2 replies to this topic

#1 Margerita

Margerita

    member

  • Active Members
  • Pip
  • 7 posts
1
Neutral

Posted 22 September 2013 - 03:12 PM

Hello!

 

Does anyone know what medium should be used to set up GH teratocarcinoma cell line? I tried to look this information up in several publications which forwarded me to other resources but the search proved unsuccessful each time. I also contacted several companies that sell different cell lines and they could not help me either.

I tried growing them in RPMI 1640 with FCS and Pen/Strep last week but they died on day 3.

Any advice would be highly appreciated.

 



#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,597 posts
384
Excellent

Posted 22 September 2013 - 04:03 PM

The usual way to go about this is to ask - Where (who from?) did you get the GH cell line from?  If you know this, then you should be able to ask them which medium they used and the other conditions necessary.

 

As far as I can tell, these are the original papers (in books), if you can find those, they should tell you:

 

KURTH,R.,LOWER,R.,LOWER,l.,HARZMANN,R.,PFEIFFER,R.,SCHMIDT,C.G.,FOGH,J.&FRANK,H. (1980). Oncovirus synthesis in human teratocarcinoma cultures ....
pp. 835-846. Edited by M. Essex,G.J.Todaro&H.zurHausen. NewYork:Cold Spring Harbor Laboratory.
 
and
 
LOWER,J.,LOWER,R.,STEGMANN,J.,FRANK,H.& KURTH, R.(1981 B).Retrovirus particle production in three of four human teratocarcinoma cell lines. In Modern Trends in Human Leukemia, vol. 4, pp. 541-544. Edited R.Neth,R.C.Gallo,T.Graf & K.Mannweiler. Berlin: Springer-Verlag


#3 Margerita

Margerita

    member

  • Active Members
  • Pip
  • 7 posts
1
Neutral

Posted 23 September 2013 - 02:44 PM

Thank you very much for your help. I have found the relevant information :)






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.