So Vmax is the maximal rate of a reaction. Is this normalized to a certain amount of enzyme?
What unit of enzyme amount is Vmax standardized to?
Posted 26 September 2013 - 11:44 AM
It is proportional to the concentration of the enzyme. The first few lines of the wiki article here will help: http://en.wikipedia.org/wiki/Michaelis%E2%80%93Menten_kinetics
But when they report the Kms of different enzyme/subtrates, it has to be standardized to a specific enyzme concentration in some way, no? The Km will be lower if you have 10x more enzyme
Posted 26 September 2013 - 02:34 PM
IIRC, Vmax should be under saturating conditions, so the rate won't change, the only thing that should change is how quickly the reaction will reach Vmax .