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What unit of enzyme amount is Vmax standardized to?


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#1 Ahrenhase

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Posted 22 September 2013 - 02:37 PM

So Vmax is the maximal rate of a reaction.  Is this normalized to a certain amount of enzyme? 



#2 bob1

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Posted 22 September 2013 - 04:06 PM

It is proportional to the concentration of the enzyme.  The first few lines of the wiki article here will help: http://en.wikipedia....Menten_kinetics



#3 Ahrenhase

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Posted 26 September 2013 - 11:44 AM

It is proportional to the concentration of the enzyme.  The first few lines of the wiki article here will help: http://en.wikipedia....Menten_kinetics

But when they report the Kms of different enzyme/subtrates, it has to be standardized to a specific enyzme concentration in some way, no?  The Km will be lower if you have 10x more enzyme



#4 bob1

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Posted 26 September 2013 - 02:34 PM

IIRC, Vmax should be under saturating conditions, so the rate won't change, the only thing that should change is how quickly the reaction will reach Vmax .






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