I am having a terrible time trying to get the WB against the CD44 protein to work. The antibody I am using recognizes CD44, and I could make it work two times but conditions never repeat!! Sometimes it works beautifully and other times not at all, it does not see anything. I know I have cell lysates since I am using other cell protein as control and it is there. Did anybody have this or similar issue and could resolve it? Or do you have any ideas of why is this happening?
Thank you very much!!