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qPCR result of pooled samples

realtime pcr pooled sample

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#1 RMF



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Posted 20 September 2013 - 05:17 AM


Here is my question in one line.

Does pooling samples produced a sum of sample copies or a mean of sample copies or something else?

Here is a more descriptive version.

A sample with 100 copies of something is analysed using real-time PCR workflow and I finally get 100 copies as the answer. Now I take two samples (assume both has 100 copies each) and pool them together and analyse them. Will my result be 100 copies or 200 copies? So I could pool 5 or 10 samples, and would I get mean, sum or something else?



Edited by RMF, 20 September 2013 - 05:21 AM.

#2 theo22



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Posted 23 September 2013 - 03:24 AM

If you pool 2 samples for qPCR and you perform the reaction identical to the unpooled sample, the result would be the average copynumber, not the total. So 100 in your example.

Also tagged with one or more of these keywords: realtime pcr, pooled sample

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