Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Amount of EDTA


  • Please log in to reply
4 replies to this topic

#1 wdyeo

wdyeo

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 54 posts
4
Neutral

Posted 18 September 2013 - 01:57 PM

Dear all, 

I made a silly big mistake by adding twice more the amount of EDTA for the MES buffer...

Will it has much affect on it?

I do not have MES and other chemicals to add to make the amount equivalent for the extra EDTA :(

 



#2 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,410 posts
234
Excellent

Posted 18 September 2013 - 04:57 PM

If the EDTA is a normal concentrations such as 1 mM, then it will little effect.



#3 wdyeo

wdyeo

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 54 posts
4
Neutral

Posted 19 September 2013 - 02:08 AM

Hi, 

Suppose i use 1mM of EDTA for 500 mL of 20x MES buffer.

But i use 1 mM of EDTA for 250 mL of 20x MES buffer.

Will it affects? :(



#4 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,410 posts
234
Excellent

Posted 19 September 2013 - 04:36 AM

No.



#5 wdyeo

wdyeo

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 54 posts
4
Neutral

Posted 21 September 2013 - 03:30 AM

Ok.

Thanks alot :D






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.