Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Adshuttle vectors and adenoviruses


  • Please log in to reply
1 reply to this topic

#1 Vector1211

Vector1211

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 18 September 2013 - 07:42 AM

Does anyone have any experience working with Adshuttle vectors? Can someone please explain how an Adshuttle vector works in terms of producing adenovirus?

 

Here is what I need to do: Clone X sequence from a retroviral vector into an Adshuttle vector with a MCS and a Rat insulin promoter. I essentially need to create adenovirus to be able to do this but I'm not very familiar with the process and what I need to look for before I begin. Do I simply start with amplifying my sequence from the retroviral vector, following by restriction enzyme digest and the cloning process?

 

Any help is much appreciated! 



#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,664 posts
396
Excellent

Posted 19 September 2013 - 03:38 PM

That sounds about right - the adshuttle vector will just contain two sequences of DNA from adenovirus (and hopefully a reporter gene too) which can be used for recombination - your gene will go between these two so that during the recombination event, your gene will be swapped over too.






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.