Does anyone have any experience working with Adshuttle vectors? Can someone please explain how an Adshuttle vector works in terms of producing adenovirus?
Here is what I need to do: Clone X sequence from a retroviral vector into an Adshuttle vector with a MCS and a Rat insulin promoter. I essentially need to create adenovirus to be able to do this but I'm not very familiar with the process and what I need to look for before I begin. Do I simply start with amplifying my sequence from the retroviral vector, following by restriction enzyme digest and the cloning process?
Any help is much appreciated!