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How long can mycobacteria tuberculosis culture survive in 4 degrees incubation?

mycobacteria survivality stock preparation

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#1 anhardanial87

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Posted 17 September 2013 - 07:58 PM

how long can mycobacteria tuberculosis culture survive in 4 degrees incubation and what simple method is used to keep Mtb in -80 degrees as stock? Thanks



#2 chaghaleh

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Posted 24 September 2013 - 01:12 PM

http://www.ncbi.nlm..../pubmed/6799264



#3 metionina

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Posted 30 September 2013 - 09:49 AM

You can keep them at -80C in 50% glycerol for many months (even years). Don't keep them at -20C.



#4 tretol

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Posted 05 October 2013 - 09:34 AM

why not, will be also frozen at -20°C ?



#5 phage434

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Posted 05 October 2013 - 12:58 PM

Cultures in 50% glycerol will not freeze at -20, and are not stable at that temperature.



#6 metionina

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Posted 06 October 2013 - 11:30 AM

I was used to keep E.coli culture at -20C and I never had problems. I did that with different mycobacterial strains: sometimes it was fine, sometimes they didn't grow after thawing. They I began to keep mycobacteria at -80C and every times they were perfectly viable.






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