I want to prepare c kit ligand conditioned medium from CHO cell. Does anyone has protocol for this method?
Another question, I tried to grow my CHO cell from stock cell line keeped in liquid nitrogen since 2004 but I found that more than 70% of cells did not attach on 10" dish surface.
The remaining cells look unhealthy. I am not sure that it causes from too long storage or not?
Please advise me
Edited by tancha, 16 September 2013 - 10:14 PM.