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c kit ligand conditioned medium from CHO cell

CHO cell

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#1 tancha

tancha

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Posted 16 September 2013 - 10:13 PM

Hi all,

I want to prepare c kit ligand conditioned medium from CHO cell. Does anyone has protocol for this method?

Another question, I tried to grow my CHO cell from stock cell line keeped in liquid nitrogen since 2004 but I found that more than 70% of cells did not attach on 10" dish surface.

The remaining cells look unhealthy. I am not sure that it causes from too long storage or not?

 

Please advise me


Edited by tancha, 16 September 2013 - 10:14 PM.






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