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hybridization probe

rt-pcr

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#1 fd_HeLiX

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Posted 16 September 2013 - 07:18 AM

I will use lightcycler 2.0. to detect and SNP with hybridization probes. I designed the probes but I couldn't choose fluorescent dyes, and don't know the criterias. Could you please help me...



#2 Curtis

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Excellent

Posted 17 September 2013 - 10:00 PM

http://www.lifetechn...and-rt-pcr.html

 

or check here for more info

 

http://www.protocol-..._PCR/index.html

 

FAM, JOE and Rhodamine are quite popular







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