I am planning to collect bovine tissue samples at an abattoir and was wondering what medium would be best to collect the samples in. I then want to mince and digest these samples and try to establish primary cell cultures. I did some reading and found all kinds of combinations of media, buffer, antibiotics and fungicides being used, but nothing specific so far. I remember in my old lab they used Hanks medium to collect human samples from the pathology lab. But as it won't be that sterile in the abattoir I was thinking of supplementing the medium with pen/strep.
Does anyone have any advice on antibiotic concentrations or media/buffer I should use in my transport medium, or even better a protocol for tissue collection and further processing in cell culture?
Thank you very much in advance,