Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

instability index of expressed protein

Instability index: protein expressed

  • Please log in to reply
1 reply to this topic

#1 sophia613

sophia613

    member

  • Active Members
  • Pip
  • 5 posts
1
Neutral

Posted 15 September 2013 - 09:35 PM

Recentl I am busy expressing a membrane protein ,my expression vector is pET32a and pET28a,but it can`t be expressed.The instability index (II) is computed to be 55.64
This classifies the protein as unstable.I get confused,it really matters?


 



#2 dimensionsbio

dimensionsbio

    member

  • Active Members
  • Pip
  • 16 posts
1
Neutral

Posted 27 September 2013 - 10:45 AM

In general, predictive calculations can be helpful when figuring out the best strategy to express a protein, but there is still no substitute for experimental data.  So does it matter?  Not really.  What's more important in your case is probably, 1) where you are targeting the expression of this membrane protein and 2) the manner in which you determine protein expression levels.






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.