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difference between an EST and a transcript sequence


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#1 Bioscientist

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Posted 14 September 2013 - 01:39 PM

Hi everyone

 

Can any one tell me what is the difference between an EST and a transcript sequence both derived by next-generation sequencing?

 

Thanks

 

Confused



#2 pcrman

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Posted 14 September 2013 - 06:47 PM

ESTs are not derived from next-g sequencing, but from single pass sequencing of cDNA clones. I am not sure what you mean by "transcript sequence".  If they are from next-g sequencing, it could mean any transcribed RNA sequence. 






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