I need to add cytokines to my cell cultures and in some protocols say I should add certain amount of cytokines per number of cells and in other protocols only refer to cytokines concentration in the culture.
If I consider cytokine concentration it looks it is irrelevant the cell number I have in the culture. But maybe for stimulation with cytokines it is in fact important the amount of cytokines per number of cells.
I would appreciate your comments! thanks!