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Plasmid Purification Using an Alkaline Lysis

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#1 Phoebe Dinh

Phoebe Dinh


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Posted 14 September 2013 - 07:28 AM

think about the procedure you used what could have caused nicked and coiled form of the plasmid to be present?

#2 bob1


    Thelymitra pulchella

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Posted 15 September 2013 - 12:45 AM

This is obviously homework - so a bit of input from you before we answer would be good.


What do you know about alkaline lysis preps? What are the steps and what does each step do?

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