Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Plasmid Purification Using an Alkaline Lysis


  • Please log in to reply
1 reply to this topic

#1 Phoebe Dinh

Phoebe Dinh

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 14 September 2013 - 07:28 AM

think about the procedure you used what could have caused nicked and coiled form of the plasmid to be present?



#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,522 posts
371
Excellent

Posted 15 September 2013 - 12:45 AM

This is obviously homework - so a bit of input from you before we answer would be good.

 

What do you know about alkaline lysis preps? What are the steps and what does each step do?






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.