What are the effects of using collagenase and dispase to digest tissue into cells?
Digestion using Collagenase and dispase
Posted 26 September 2013 - 07:30 AM
There are many enzymes that we use to digest tissue. Some are more effective than others and can be used in combination.
In answer to your specific question. There are 4 types of collagenase, please refer to the link below:-
There are 2 types of Dispase, again please refer to the link below:
Generally speaking researchers will follow a published protocol. However even in these circumstances you will have to optimise conditions i.e. concentration of enzyme, length of exposure, temperature, rate of shaking etc
Hope this is useful