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Digestion using Collagenase and dispase


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#1 kk89

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Posted 13 September 2013 - 10:32 AM

What are the effects of using collagenase and dispase to digest tissue into cells? 



#2 rhombus

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Posted 26 September 2013 - 07:30 AM

Dear KK89,

 

There are many enzymes that we use to digest tissue. Some are more effective than others and can be used in combination.

 

In answer to your specific question. There are 4 types of collagenase, please refer to the link below:-

 

http://www.worthingt...com/cls/CLS.pdf

 

There are 2 types of Dispase, again please refer to the link below:

 

http://www.worthingt...ochem.com/DISP/

 

Generally speaking researchers will follow a published protocol. However even in these circumstances you will have to optimise conditions i.e. concentration of enzyme, length of exposure, temperature, rate of shaking etc

 

Hope this is useful

 

Kindest regards

 

Uncle Rhombus






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