I need an advice how to calculate final cytokine concentration in tissue culture in pg/ml/mg. I cultured tissue explants about 200mg per 700ul media. Then I collected supernatants and measured tnf production by ELISA (100ul of supernatant per well). My final result from this assay is in pg/ml but I need to calculate them with relation to mg of tissue.. How can I do that?
I tried to express my tissue culture concentration as 280mg/ml - but what I should do after that? Should I devide my ELISA result or multiply? A little bit confused..