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DiI staining lipoproteins


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#1 julnobody

julnobody

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Posted 11 September 2013 - 11:46 PM

Hi,

 

I am planning to stain lipoproteins using DiI dye, most protocols I've found so far use lipoprotein-deficient serum (LPDS) to resuspend the lipoproteins for staining.

 

I was wondering if anyone had used an alternative to the LPDS to use as staining buffer, other than PBS/BSA (The lipoproteins I have are purified in saline solution) ?

 

Thank you  






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