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pH a Buffer

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#1 buddd3



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Posted 11 September 2013 - 06:14 AM

Why is it necessary to only add about half or 3/4 of the diluent for a buffer, pH, and then add the rest? Why can't I add the full amount and then pH? The buffer consists of dry milk and PBS, with a base increasing the pH. Thank you!

#2 mdfenko


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Posted 11 September 2013 - 06:21 AM

because you will go over the volume for which your components were calculated. therefore, your concentrations will be off.

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