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RNA and DNA extraction and PCR control


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#1 gavri

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Posted 20 April 2004 - 04:20 AM

Hello!
Im about to setup a diagnostic real-time PCR for detection of RNA and DNA viruses. Now Im looking for suitable internal control for RNA and DNA extraction and PCR as well. The sample material will be tissue, blood or serum. Do you have any suggestion?

Thank you for your help

#2 phdconsult

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Posted 20 April 2004 - 04:48 AM

Gavri

The best control will be blood/serum sample spiked with various dilutions of viral DNA/RNA and processed the way you would process your samples.

Good Luck and God Bless




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