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Can the malondialdehyde assay be used to determine the concentration of a lipid

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#1 Cucumber



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Posted 09 September 2013 - 10:10 PM

Hi everyone,


I am attempting to generate a lipid peroxide (squalene peroxide) from squalene by dissolving it in heptane solvent at a concentration of 1mg/ml and exposing it to UVA. According to literature, only about 60% of squalene will dissociate. My problem is I need to determine the concentration of squalene peroxide in this mixture. Other researchers have used the HPLC or the mass spec, but because access to these equipment is hard for me, I was wondering if there was an assay to quantify lipid peroxides.


My question is: can the MDA assay be used to determine indirectly the concentration of squalene peroxide? My concern is that MDA is an end product of lipid peroxidation, whereas squalene peroxide is an intermediate product, so I have no idea how to correlate the 2. Any other suggestions greatly appreciated!


Thank you for your help :)

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