I am trying to use polyethylene glycol (PEG), MW 8000, to coat a tissue culture substrate to decrease cell adhesion. The substrate is patterned similarly to the AggreWell plates from StemCell Technologies (which have little 800 um microwells or indentations that force cells to aggregate into spheres).
So my question is how to evenly coat the surfaces of these tiny microwells with a thin layer of PEG. So far, I have tried allowing a 10% solution of PEG in dH2O to evaporate, but the deposited PEG appears to be extremely uneven (see attached photo).
Any help is appreciated!!