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Question: How to differentiate siRNAs and mature miRNAs in plant by Northern blo


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#1 Song Guo

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Posted 07 September 2013 - 09:11 PM

After virus infection, the gene silencing will generate lots of virus-siRNAs, but also there are some host mature miRNAs in the host plant, how to differentiate the virus-generated-siRNAs and host mature miRNAs by Northern blot? 



#2 pcrman

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Posted 08 September 2013 - 10:21 AM

If they are different in sequence, Northern should be able to tell them apart. 



#3 Song Guo

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Posted 08 September 2013 - 07:39 PM

If there are different in sequence, Northern should be able to tell them apart. 

Thanks for your reply, if the sequences are different, then I should using different probes to detect siRNAs and mature miRANs separately, right? But how come if the virus can also generate miRNAs? 

Thanks. 



#4 pcrman

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Posted 08 September 2013 - 09:26 PM

Yes, it is the probe which tells them apart, not their size. 






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