After virus infection, the gene silencing will generate lots of virus-siRNAs, but also there are some host mature miRNAs in the host plant, how to differentiate the virus-generated-siRNAs and host mature miRNAs by Northern blot?
Question: How to differentiate siRNAs and mature miRNAs in plant by Northern blo
Posted 08 September 2013 - 10:21 AM
If they are different in sequence, Northern should be able to tell them apart.
Posted 08 September 2013 - 07:39 PM
If there are different in sequence, Northern should be able to tell them apart.
Thanks for your reply, if the sequences are different, then I should using different probes to detect siRNAs and mature miRANs separately, right? But how come if the virus can also generate miRNAs?
Posted 08 September 2013 - 09:26 PM
Yes, it is the probe which tells them apart, not their size.