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DNA storage degradation


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#1 ddomingof

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Posted 06 September 2013 - 02:36 PM

Hello,

 

I have to do a work for a class about the stability of DNA in Drosophila melanogaster.

We would like to know how many time can be frozen the DNA of one sample without any kind of degration.

The same for one solution in a buffer between diferents temperatures.

 

To check that, we will do a PCR assuming that if we don't get amplification, the DNA has been degradated.

 

What temperatures would you use? Any other ideas?

 

Thank you.



#2 bob1

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Posted 06 September 2013 - 06:43 PM

here... hint: a little bit of input from you will help us know how much you know doing the research yourself, helps you learn!

 

As for the PCR - temps depend on the primers.  Are you sure that PCR is a good way to assess DNA degradation?






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