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Largest viral insert?


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#1 unknownphd

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Posted 17 April 2004 - 01:42 PM

I was wondering how large of an insert I can put into a viral plasmid? I plan to put a 7kb insert into a 5.1kb viral plasmid (pBabe-puro). I'm worried that this large insert won't package correctly.

Does anyone have any experience with this?

Thanks!

Edited by unknownphd, 17 April 2004 - 02:43 PM.


#2 Antigen

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Posted 19 April 2004 - 07:04 AM

I had successful experience of cloning 12kb insert into pGem (3kb). Still think it is not limit.




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