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Tet-On inducible gene expression


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#1 Rajpoot

Rajpoot

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Posted 06 September 2013 - 04:46 AM

Hi All,

I plan to express my gene of interest in this tet-on pLenti CMVtight Hygro DEST (w769-1) [http://www.addgene.org/26433/]. Will my gene of interest express in Doxicyline inducible manner if I clone blunt ended CDS into BSTZ17I linearized vector?

Another silly question, can third generation tetracycline transactivator induce expression from sencod generation Tet-on promoter?

 

Thanks in advance
 

 

 

Attached Thumbnails

  • pLenti CMVtight Hygro DEST (w769-1) Map.png





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