Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Disappeared bands after the addition of Sigma P2714

Usage of Prot. inhibitor Sigma P2714

  • Please log in to reply
1 reply to this topic

#1 domwiledu



  • Members
  • Pip
  • 1 posts

Posted 05 September 2013 - 11:40 PM

Hey  I am kinda new in proteomics things and I need a whole lot of help here...

the thing is ... we are now trying to extract proteins specific ta target mango var.  using SDS-PAGE, we are using the acetone precipitation method to extract our proteins. Before we add Protease inhibitor mix we had a lot of bands that appeared but in the next runs after the addition of the inhibitor we had an ultra clear GEL! Zero Bands!  How should we USE the Protease inhibtor mix? 

#2 mdfenko


    an elder emeritus

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,259 posts

Posted 06 September 2013 - 03:45 AM

protease inhibitors should be introduced with the medium used for extraction and continued in all subsequent media until the protein of interest is purified.

talent does what it can
genius does what it must
i do what i get paid to do

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.