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PCR problem


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#1 medbio

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Posted 05 September 2013 - 06:59 AM

Hi,

 

When I use above 15 DNA samples, my PCR doesn' t work but when I use below 15 (same DNA), the pcr is perfect. I use invitrogen' s taq dna polymerase, dntp. According to you what is the problem?



#2 hobglobin

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Posted 05 September 2013 - 11:38 AM

some more details would be good. Are you using a mastermix?


One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.

#3 medbio

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Posted 05 September 2013 - 11:43 AM

Yes, I use mm everytime.






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