I'm new here so I apologize if I am writing in wrong forum.
My question is:
I have tissue samples of different organs and different animals.
I'm looking for the ratio of a gene and its alternatively spliced form (isoform).
Does anyone have any idea with which method to calculate the ratio. I have Ct values that I've gotten them with qPCR. I am using the endogenous controls.
If I go with the method 2^(-delta delat Ct) I need a calibrator and I don't know if I can use any of the samples because I don't know what is the state 0 (the isoform is present everywhere in every tissue).
- want to calculate ratio between two forms of same gene (wild type and its isoform).
- I have tissue samples (can't gen cell cultures or anything)
- working with qPCR
Thank you all for help.