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Sodium Acetate pH 5.2 or 5.5 for DNA precipitation

DNA sodium acetate pH precipitation

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#1 My5pam

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Posted 05 September 2013 - 04:21 AM

I have seen a protocol calling for sodium acetate at pH 5.2 for use with ethanol and glycogen when precipitating DNA into a pellet.

 

However, I used a commercial socium acetate which I just realized is pH 5.5.

 

How will this influence the precipitation of my DNA?

 

Thanks!



#2 Curtis

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Excellent

Posted 06 September 2013 - 08:48 AM

Not much, go ahead.





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