Actually, I performed the IF on control cells and cells transfected with siRNA. I don't know how much the protein was deleted.
The problem is that signal in 'siRNA' cell is really strong, sounds specific. But in control cells, the signal is weaker weaker. The morphology seems the same.
Because the number of cells on the coverslips of cell transfected is fewer (90%) in comparison with the number of control cells.
If the quantity of antibody is the same, could the antibody react and fix more in a smaller number of cells?
Sorry for this stupid question.
Thanks in advance.