Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

What is the membrane made of in DNA purification kits


  • Please log in to reply
3 replies to this topic

#1 Ahrenhase

Ahrenhase

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 164 posts
10
Good

Posted 03 September 2013 - 10:57 AM

is it silica or cellulose acetate



#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,663 posts
394
Excellent

Posted 03 September 2013 - 12:30 PM

Depends on the kit - Promega's wizard kits had diatomaceous earth (essentially silicon dioxide) as the filter, but other kits use formulations of glass.  I don't know if there are any that use cellulose acetate, but there could well be.



#3 Ahrenhase

Ahrenhase

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 164 posts
10
Good

Posted 03 September 2013 - 01:15 PM

Depends on the kit - Promega's wizard kits had diatomaceous earth (essentially silicon dioxide) as the filter, but other kits use formulations of glass.  I don't know if there are any that use cellulose acetate, but there could well be.

These are the cellulose acetate columns I was referring to

http://www.sigmaaldr...ng=en&region=US



#4 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,437 posts
241
Excellent

Posted 03 September 2013 - 03:51 PM

Those are filters. I don't think they bind DNA effectively. You could use them with diatomaceous earth or silica which is filtered by them.






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.