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Raji Cell transfection

raji

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#1 Avinanda Banerjee

Avinanda Banerjee

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Posted 03 September 2013 - 12:25 AM

HI All

 

I am culturing raji cell line in RPMI media supllimented with 10% FBS, we all know it is a suspension cell but my cells are sticking to the surface( Nunc T25 flask) n need to be trypsinized for passaging. is it pretty normal condition or worng please suggest. can i transfect raji cell with lipo2k or i need to do electroporation for transfection.






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