I would like to ask for you help. I have my iPS cell culture on inactivated mouse embryonic fibroblasts, but we would like to switch to, serum- and feeder-free system from StemCell: "TeSR."
It is well defined, I understand why they use some components like bFGF and so on... But cant reach an answer, why they use these components in medium:
-GABA (γ-Aminobutyric acid)
-Lithium Chloride (I guess this is used for salt concentration)
So... any help would be great! I know that this isnt essential knowledge for correct usage of media. I am jut too curious to don't know why they use these components