i have working on rbohD , after drought stress i cheked the exporession of this gene in my plant.
i have two problem
1. I got High CT in my negetive husekeeping gene (GAPDH) but after check in agarose i didnt see any thing. I am worry about my final result after normalosation with this house keeping gene.
2. sometimes double melting curve in my specific gene only in one accession.