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AFLP band elution and reamplification problem

AFLP molecular markers

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#1 saripalligautam



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Posted 01 September 2013 - 08:15 PM

I am trying to elute some polymorphic bands from my AFLP dried PAGE (autoradiograph) gel.But after elution when i reamplify my eluted band, the product size is half of the expected size.If i elute 500 bp, the product size after reamplification is 250 bps.Can someone extend some suggestions in this regard?

Also tagged with one or more of these keywords: AFLP, molecular markers

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