Has anyone tried this and had success at this? I was interested in trying this for some brain samples.
New CLARITY system
Posted 08 February 2014 - 10:23 AM
Yes. I've tried it and had mixed/poor results.
If you follow the protocol provided on the clarity website, you'll probably get a clear brain. However, you'll need to spend time optimizing the osmolarity of the solutions you use. I've noticed that transferring the sample from clearing solution to imaging solution induces a rapid condensation of the tissue, which will destroy any morphology that you see under the microscope. This is particularly troublesome if you're using a glycerol solution for imaging.
That said, I was able to identify cell bodies and projections on a Thy1-YFP mouse using a high NA objective and confocal system. We captured a neat ~600 micron thick section and the 3D reconstruction of the z-sections turned out fairly well. I hope to get back to it, it has potential.
I'd suggest trying it on small sections first and scaling up to the whole brain later, to optimize/minimize the osmotic damage to your tissue.
Cheers and good luck.