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Suggestion for anti CD44 antibody for WB and IP?

CD44 U2OS IP

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#1 Guada

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Posted 29 August 2013 - 11:14 AM

Hello everyone,

I am having issues to find antibodies that work for WB and IP of CD44 in U2oS cells. The contents of CD44 in this cells should be high and I am able to observe it by IF but the antibody we got does not even give a band in WB studies. Does anyone have any suggestion?

Thank you very much

Maria



#2 bob1

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Posted 29 August 2013 - 05:54 PM

Hmmm, there are two options here - either your antibody only recognizes the native form of CD44 (SDS-PAGE gels are denaturing), or the signal you get on the IF is non-specific.  Check the antibody data-sheet, this may give you some indication.

 

You best bet is to check the literature and see which antibodies people have used.






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