I am using thrombin to remove a GST tag from my fusion protein. I am doing a time-course to check how long to incubate my protein with thrombin. I decided to add samples straight to my sample loading buffer at the appropriate time point and I will load the gel the next day. The final concentration of beta-mercaptoethanol and SDS in the sample buffer will be 5% (710mM) beta-ME and 10% SDS. I have read that 10mM beta-ME and 0.01% SDS do not inactivate thrombin. Does anyone know if 710mM beta-ME and 10% SDS will completely inactivate the thrombin?