I am looking for a method to induce oxidative stress for a longer period of time (24-72h) in HEK cells. As we want to measure antioxidative response, the ROS-inducer should not increase ROS-Levels by downregulating antioxidative response. Furthermore it should not directly affect mitochondria (indirectly by the means of ROS is not avoidable). So for example Antimycin A is not a good opportunity.
Xanthin/Xanthinoxidase system seems to be a good possibility, but I have read that there is a feedback inhibition so it is only usable for approximatelly 30 min.
Any ideas what else could be used?
Sorry for this quite open question, so far I am not a specialist in ROS.