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Inducing oxidative stress


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#1 coni

coni

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Posted 29 August 2013 - 04:42 AM

I am looking for a method to induce oxidative stress for a longer period of time (24-72h) in HEK cells. As we want to measure antioxidative response, the ROS-inducer should not increase ROS-Levels by downregulating antioxidative response. Furthermore it should not directly affect mitochondria (indirectly by the means of ROS is not avoidable). So for example Antimycin A is not a good opportunity.

Xanthin/Xanthinoxidase system seems to be a good possibility, but I have read that there is a feedback inhibition so it is only usable for approximatelly 30 min.

Any ideas what else could be used?

 

Sorry for this quite open question, so far I am not a specialist in ROS.






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